The Center for Clinical Diagnostics includes labs that provide diagnostic testing services in support of a variety of medical specialties and patient care at ¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ·.
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They include:
Cardiac Diagnostic Lab in Delaware
We perform basic science research on cardiac tissue, including the investigation of heart injury, assessment of the body’s response to that injury and the development of heart failure. The lab is working to validate and implement a new test panel for an increase in test volume.
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Contact
Robert E. Akins, Jr., PhD
(302) 651-6811
robert.akins@nemours.org
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Cardiac Diagnostic Lab
¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Children’s Hospital, Delaware
1600 Rockland Road, ARB-283
Wilmington, DE 19803
Diagnostic Pathology Lab in Orlando
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Diagnostic Pathology Lab in Orlando
This lab works on developing quantitative methods for surgical pathology and diagnostic decision-making, especially involving diagnostic spectroscopy ("optical biopsy") and quantitative microscopy. We’re developing simple and practical optical and quantitative methods as adjunct diagnostic tools in everyday practice of diagnostic pathology.
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Contact
(407) 650-7807
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Diagnostic Pathology Lab
¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Children’s Hospital
6535 ¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Parkway
Orlando, FL 32827
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Gastroenterology Clinical Lab in Delaware
This lab is a CLIA (Clinical Laboratory Improvement Amendments)-certified lab that provides services to develop research projects for industry and research institutions. Current research collaborators include Mayo Clinic, Fla.; Cooper University Hospital, N.J.; and Digestive Care, Inc., Pa. We also accept samples for research studies and clinical trials.
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We offer the following tests:
Disaccharidase Determination, Small Bowel Biopsy
The activity of disaccharidases (including lactase, maltase, sucrase, palatinase and glucoamylase) can be determined from an intestinal biopsy with approximately 2 to 5 mg of wet weight.
If you’re submitting a sample for testing, please:
- Avoid contamination with traces of the fixative used for surgical pathology biopsies.
- Upon collection, place the specimen in a small, tightly capped plastic tube and immediately freeze it on dry ice or stored in a freezer at -20°C to -70°C (no automatic defrost cycles). The biopsy should not be placed on gauze or a toothpick, nor should any solutions be added.
- Ship the sample with enough dry ice to remain frozen. Enzyme activity may be affected if the sample thaws.
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Pancreatic Enzyme Activities, Duodenal Aspirate
Amylase, lipase, trypsin, chymotrypsin and elastase activities can be determined from duodenal fluid. A minimum of 0.2 ml of fluid is required to analyze the five enzymes, protein content and pH. The sample can be a single fluid or multiple fluids.
If you’re submitting a sample for testing, please:
Place the fluid in a small, tightly capped, plastic tube. Keep the sample frozen and ship on dry ice.
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Gastric Pepsin Assay
Gastric pepsin detected in the airway of patients is a specific and sensitive marker to assess pulmonary aspiration. The GI Clinical Lab at ¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· offers a sensitive, reliable enzymatic test to detect gastric pepsin in the airway samples. The assay has high specificity as it detects pepsin A that is exclusively expressed in the gastric mucosa.
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- To collect tracheal secretion: Attach a LUKI trap to a suction catheter, pass the catheter down the endotracheal tube and apply suction to the catheter as the catheter is withdrawn. If the secretion is too thick to be withdrawn, instill saline (2 cc) to the tracheal tube prior to aspirating the secretion.
- To collect bronchial secretion: Bronchial secretion can be collected using a standard collection protocol. Collect 1-2 cc bronchial washing fluid in a plain specimen tube. Please avoid over-diluting the secretion with a large amount of saline during the collection. A minimum of 0.5 cc fluid is required to determine gastric pepsin activity, protein content and pH. The airway fluid should be transferred to a small, tightly capped plastic tube and frozen immediately after collection. Keep sample frozen and ship on dry ice.
Please contact us for more information and individual test prices.
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Contact
Paul T. Fawcett, PhD
(302) 651-6826
paul.fawcett@nemours.org
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Gastroenterology Clinical Lab
¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Children’s Hospital, Delaware
1600 Rockland Road, ARB-250
Wilmington, DE 19803
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Clinical Immunology Lab in Delaware
The Clinical Immunology Lab is a College of American Pathologists (CAP)-certified lab that specializes in the serologic detection of Lyme disease and autoimmune disorders.
The lab currently provides immunodiagnostic testing services for several medical divisions, especially Gastroenterology and Rheumatology. These areas of study were identified in response to the need for improved ability to diagnose and formulate a prognosis for related diseases. The lab’s approach to these investigations emphasizes the role of the immune system as both an effector/modulator and as an indicator of disease in these disorders.
We provide diagnostic testing services as requested by our hospital physicians. Available tests include assays for auto-antibodies and for infectious diseases. We have developed a series of in-vitro culture techniques and serologic assays that enable us to examine cells and blood from patients with inflammatory joint diseases (in an effort to determine the cause of this class of disease).
Paul T. Fawcett, PhD, has been the head of the research and clinical immunology laboratories since 1986. He also is a member of the Institutional Review Board (IRB).
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Our team also includes:
Lisa Loftus, BS, MT(ASCP)
Victoria L. Maduskuie, BS, MT (ASCP)
Marjorie Postell, BT, MLT
Timothy G. Stetson
Pamela Rullo, administrative secretary
Contact
Paul T. Fawcett, PhD
(302) 651-6826
paul.fawcett@nemours.org
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Clinical Immunology Lab
¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Children’s Hospital, DelawareÌýÌý
1600 Rockland Road, ARB-250
Wilmington, DE 19803
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Transplant Molecular Diagnostics Lab in Delaware
Transplant Molecular Diagnostics is a CLIA-certified lab that performs clinical testing to monitor transplant and other immunocompromised pediatric patients. Most of our tests incorporate quantitative real-time PCR (polymerase chain reaction) technology that rapidly links amplification with detection and quantification of viral DNA.
Currently, our real-time PCR tests detect viral DNA loads that may be indicative of an active infection that may warrant the institution of antiviral therapies and/or a decrease of immunosuppressive therapies. Antiviral therapy is instituted upon viral DNA detection, followed by close monitoring of quantitative viral titers to assess and maintain the balance between immunosuppression and anti-infective therapies.
Available tests include:
- The detection and monitoring of BK polyomavirus (BKV) quantitative real-time PCR (polymerase chain reaction)
- Epstein-Barr Virus (EBV) Quantitative Real-Time PCR
- Cytomegalovirus (CMV) Quantitative Real-Time PCR. Monitoring CMV DNA levels in blood by quantitative real-time PCR in these patients may allow timely recognition of virus reactivation and permit installment and assessment of antiviral treatment (ganciclovir) and/or a decrease of immunosuppressive therapies.
- Adenovirus (AdV) Quantitative Real-Time PCR. Monitoring AdV DNA levels of blood by quantitative real-time PCR in these patients may allow timely recognition of virus reactivation and permit installment and assessment of antiviral treatment (IVIG and also cidofavir in severe cases) and/or decrease of immunosuppressive therapies. Depending on a patient’s symptoms, other specimens may be tested, such as respiratory and stool samples.
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For ordering information:
Carrie Paquette-Straub, MS
Clinical Research Assistant
(302) 651-6776
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For more about the testing available:
Victoria Maduskuie, BS, MT (ASCP)
Assistant Director and Immunology Supervisor
(302) 651-6776
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Contact
Paul T. Fawcett, PhD
(302) 651-6826
paul.fawcett@nemours.org
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Research Immunology
¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾Ã¾«Æ· Children’s Hospital, DelawareÌýÌý
1600 Rockland Road, ARB-250
Wilmington, DE 19803
